FITC Plus Anti-Human VISTA Rabbit Recombinant Antibody

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• FITC Plus Anti-Human VISTA Rabbit Recombinant Antibody (FITC-98109)

VISTA Uni-rAb^® Recombinant Antibody for FC

Cat No. FITC-98109 Copied!

Clone No. 241003C8

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

C10orf54, 241003C8, B7-H5, GI24, PP2135

• 

  FC experiment of human PBMCs using FITC-98109

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human VISTA Rabbit RecAb (FITC-98109, Clone: 241003C8) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using FITC-98109

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human VISTA Rabbit RecAb (FITC-98109, Clone: 241003C8) (red) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98109 targets VISTA in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0017

  Product name: Recombinant Human VISTA/C10orf54 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 33-194 aa of BC020568

  Sequence: FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAA

• Full Name: chromosome 10 open reading frame 54
• Calculated Molecular Weight: 311 aa, 34 kDa
• GenBank Accession Number: BC020568
• Gene Symbol: VISTA
• Gene ID (NCBI): 64115
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H7M9
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.