VMO1 Polyclonal antibody

VMO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21577-1-AP

Synonyms

Vitelline membrane outer layer protein 1 homolog, UNQ6350/PRO21055

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Validation Data Gallery

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  WB analysis of HeLa using 21577-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21577-1-AP (VMO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21577-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21577-1-AP (VMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21577-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21577-1-AP (VMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 21577-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21577-1-AP (VMO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21577-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21577-1-AP (VMO1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21577-1-AP

  Immunofluorescent analysis of HeLa cells using 21577-1-AP (VMO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21577-1-AP targets VMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VMO1 fusion protein Ag16120
• Full Name: vitelline membrane outer layer 1 homolog (chicken)
• Calculated Molecular Weight: 202 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC104195
• Gene Symbol: VMO1
• Gene ID (NCBI): 284013
• RRID: AB_10860262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.