VPRBP Polyclonal antibody, PBS Only

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• VPRBP Polyclonal antibody, PBS Only (11612-1-PBS)

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  WB analysis using 11612-1-AP (same clone as 11612-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11612-1-AP (same clone as 11612-1-PBS)

  WB result of VPRBP antibody (11612-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11612-1-AP (same clone as 11612-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11612-1-AP (same clone as 11612-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 11612-1-AP (same clone as 11612-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 11612-1-AP (same clone as 11612-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11612-1-AP (same clone as 11612-1-PBS)

  IP result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11612-1-AP (VPRBP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11612-1-AP (same clone as 11612-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (11612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 11612-1-AP (same clone as 11612-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug VPRBP Polyclonal antibody (11612-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

VPRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 11612-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

DCAF1, DDB1- and CUL4-associated factor 1, EC:2.7.11.1, HIV 1 Vpr binding protein, HIV-1 Vpr-binding protein

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Product Information

11612-1-PBS targets VPRBP in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2184

  Product name: Recombinant human VPRBP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 707-1058 aa of BC022792

  Sequence: WSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

• Full Name: Vpr (HIV-1) binding protein
• Calculated Molecular Weight: 1506 aa, 169 kDa
• Observed Molecular Weight: 169 kDa
• GenBank Accession Number: BC022792
• Gene Symbol: VPRBP
• Gene ID (NCBI): 9730
• RRID: AB_2216933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y4B6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).