Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, K-562 cells, PC-3 cells, DU 145 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive IF/ICC detected in | HepG2 cells, human breast cancer tissue |
| Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66392-1-Ig targets VPRBP in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2184 Product name: Recombinant human VPRBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 707-1058 aa of BC022792 Sequence: WSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE Predict reactive species |
| Full Name | Vpr (HIV-1) binding protein |
| Calculated Molecular Weight | 1506 aa, 169 kDa |
| Observed Molecular Weight | 169 kDa |
| GenBank Accession Number | BC022792 |
| Gene Symbol | VPRBP |
| Gene ID (NCBI) | 9730 |
| RRID | AB_2881768 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y4B6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for VPRBP antibody 66392-1-Ig | Download protocol |
| IF protocol for VPRBP antibody 66392-1-Ig | Download protocol |
| IHC protocol for VPRBP antibody 66392-1-Ig | Download protocol |
| WB protocol for VPRBP antibody 66392-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VPRBP Monoclonal antibody (1A7A8), catalog number 66392-1-Ig, has 2 citations. These appear in Nature Communications and Cell Communication and Signaling. The cited research spans cancer biology: one study investigates EZH2 phosphorylation and stabilization by DCAF1/VprBP in aberrant gene silencing in colon cancer, while the other examines VprBP-driven prostate tumorigenesis through its kinase activity targeting histone H2A. Both citations use the antibody for Western blot (WB) applications in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger aberrant gene silencing in colon cancer
| ||
Cell Commun Signal VprBP drives prostate tumorigenesis by its kinase activity targeting histone H2A. |
Reviews
What customers say
The single review for 66392-1-Ig is a 5-star rating from an Immunofluorescence application. The user worked with SW620 cell xenograft tissue at a 1:200 dilution, reporting that the antibody produced a clear red signal (VprBP) with DAPI counterstaining in blue. Overall, the feedback indicates the antibody performed well for IF, delivering specific and visible staining in the tested sample.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nikhil (Verified Customer) (01-07-2022) | SW620 cell xenograft. Red colour is VprBP. Blue is DAPI.
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