VPRBP Monoclonal antibody

VPRBP Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1A7A8

Cat no : 66392-1-Ig

Synonyms

DCAF1, HIV 1 Vpr binding protein, KIAA0800, Protein VPRBP, RIP, Vpr (HIV 1) binding protein, Vpr interacting protein, VPRBP



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, K-562 cells, HEK-293 cells, PC-3 cells, DU 145 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66392-1-Ig targets VPRBP in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VPRBP fusion protein Ag2184
Full Name Vpr (HIV-1) binding protein
Calculated molecular weight 1506 aa, 169 kDa
Observed molecular weight 169 kDa
GenBank accession numberBC022792
Gene symbol VPRBP
Gene ID (NCBI) 9730
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

Protocols

Product Specific Protocols
WB protocol for VPRBP antibody 66392-1-IgDownload protocol
IHC protocol for VPRBP antibody 66392-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols