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VPS16 Polyclonal antibody

VPS16 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17776-1-AP

Publications (27)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

hVPS16, VPS16

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis using 17776-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 17776-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 17776-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 17776-1-AP
IP result of anti-VPS16 (IP:17776-1-AP, 3ug; Detection:17776-1-AP 1:500) with HeLa cells lysate 2480ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 17776-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 17776-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 17776-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 17776-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (27)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17776-1-AP targets VPS16 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12202 Product name: Recombinant human VPS16 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 177-525 aa of BC073959 Sequence: KVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK Predict reactive species
Full Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Calculated Molecular Weight	839 aa, 95 kDa
Observed Molecular Weight	95 kDa
GenBank Accession Number	BC073959
Gene Symbol	VPS16
Gene ID (NCBI)	64601
RRID	AB_2217085
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H269
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
IHC protocol for VPS16 antibody 17776-1-AP	Download protocol
IP protocol for VPS16 antibody 17776-1-AP	Download protocol
WB protocol for VPS16 antibody 17776-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-STX17 antibody (Cat# 17776-1-AP) has 27 citations spanning 24 journals, including Autophagy, Nature Communications, Molecular Cell, Cell Death & Disease, Developmental Cell, and Current Biology. Associated research fields cover autophagy, lysosomal biology, exosome/extracellular vesicle release, endocytosis, membrane trafficking and tethering, neuroprotection, cancer biology, and podocyte function. Applications include WB, IF, IP, and CoIP in human, mouse, and rat models.

Species	Application	Title
human	WB	Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes Authors - Wang Zheng View Article
human	WB	Autophagy Acetylation of STX17 (syntaxin 17) controls autophagosome maturation. Authors - Qiuhong Shen View Article
human	WB,IP	Nat Commun Deacetylated SNAP47 recruits HOPS to facilitate autophagosome-lysosome fusion independent of STX17 Authors - Fenglei Jian View Article
human	WB	Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy Authors - Shen Zhang View Article
mouse	IF	Redox Biol Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes. Authors - Guangbi Li View Article
mouse	WB	Mol Cell Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest. Authors - Ian G Ganley KD Validated View Article