CoraLite® Plus 488-conjugated VPS4B Monoclonal antibody

VPS4B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H3G7

Cat no : CL488-68443

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

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Validation Data Gallery

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  IF Staining of HeLa using CL488-68443

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 VPS4B antibody (CL488-68443, Clone: 2H3G7 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68443 targets VPS4B in IF/ICC applications and shows reactivity with Human, mouse , rat samples.

• Tested Reactivity: Human, mouse , rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VPS4B fusion protein Ag11610
• Full Name: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 444 aa, 49 kDa
• Observed Molecular Weight: ~50 kDa
• GenBank Accession Number: BC039574
• Gene Symbol: VPS4B
• Gene ID (NCBI): 9525
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)