VTI1B Polyclonal antibody, PBS Only

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• VTI1B Polyclonal antibody, PBS Only (14495-1-PBS)

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  WB analysis using 14495-1-AP (same clone as 14495-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 14495-1-AP (same clone as 14495-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 14495-1-AP (same clone as 14495-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 14495-1-AP (same clone as 14495-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14495-1-AP (same clone as 14495-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14495-1-AP (same clone as 14495-1-PBS)

  IP result of anti-VTI1B (IP:14495-1-AP, 3ug; Detection:14495-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

VTI1B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14495-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Vesicle transport through interaction with t-SNAREs homolog 1B, Vesicle transport v-SNARE protein Vti1-like 1, VTI1, VTI1 LIKE, Vti1 rp1

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Product Information

14495-1-PBS targets VTI1B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5906

  Product name: Recombinant human VTI1B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 13-232 aa of BC003142

  Sequence: KLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLLSIIILLELAILGGLVYYKFFRSH

• Full Name: vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC003142
• Gene Symbol: VTI1B
• Gene ID (NCBI): 10490
• RRID: AB_2216160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UEU0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp, which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8, and VAMP8, which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7, and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).