VTN Monoclonal antibody

VTN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2C8

Cat no : 66398-1-Ig

Synonyms

S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN



Tested Applications

Positive WB detected inhuman plasma tissue, pig plasma tissue, rabbit serum tissue
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66398-1-Ig targets VTN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit, mouse samples.

Tested Reactivity human, pig, rabbit, mouse
Cited Reactivitypig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VTN fusion protein Ag8443
Full Name vitronectin
Calculated Molecular Weight 478 aa, 54 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC005046
Gene Symbol VTN
Gene ID (NCBI) 7448
RRIDAB_2881772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

Protocols

Product Specific Protocols
WB protocol for VTN antibody 66398-1-IgDownload protocol
IHC protocol for VTN antibody 66398-1-IgDownload protocol
IF protocol for VTN antibody 66398-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIHC

Int J Mol Sci

Large-Scale Protein Analysis of Experimental Retinal Artery Occlusion

Authors - Nanna Vestergaard