VTN Monoclonal antibody

VTN Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D2C8

Cat no : 66398-1-Ig

Synonyms

S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN



Tested Applications

Positive WB detected inhuman plasma tissue, pig plasma tissue, rabbit serum tissue
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66398-1-Ig targets VTN in WB, IHC,ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VTN fusion protein Ag8443
Full Name vitronectin
Calculated molecular weight 478 aa, 54 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC005046
Gene symbol VTN
Gene ID (NCBI) 7448
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

Protocols

Product Specific Protocols
WB protocol for VTN antibody 66398-1-IgDownload protocol
IHC protocol for VTN antibody 66398-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols