Villin like Polyclonal antibody, PBS Only

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• Villin like Polyclonal antibody, PBS Only (15130-1-PBS)

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  WB analysis using 15130-1-AP (same clone as 15130-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15130-1-AP (VILL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15130-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 15130-1-AP (same clone as 15130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15130-1-AP (VILL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15130-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 15130-1-AP (same clone as 15130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15130-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat small intestine using 15130-1-AP (same clone as 15130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat small intestine tissue using Villin antibody (15130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15130-1-PBS in a different storage buffer formulation.

Villin like Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 15130-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

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Product Information

15130-1-PBS targets Villin like in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7259

  Product name: Recombinant human VILL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 334-687 aa of BC000243

  Sequence: SASTTRLFQVQGTDSHNTRTMEVPARASSLNSSDIFLLVTASVCYLWFGKGCNGDQREMARVVVTVISRKNEETVLEGQEPPHFWEALGGRAPYPSNKRLPEEVPSFQPRLFECSSHMGCLVLAEVGFFSQEDLDKYDIMLLDTWQEIFLWLGEAASEWKEAVAWGQEYLKTHPAGRSPATPIVLVKQGHEPPTFIGWFFTWDPYKWTSHPSHKEVVDGSPAAASTISEITAEVNNLRLSRWPGNGRAGAVALQALKGSQDSSENDLVRSPKSAGSRTSSSVSSTSATINGGLRREQLMHQAVEDLPEGVDPARREFYLSDSDFQDIFGKSKEEFYSMATWRQRQEKKQLGFF

• Full Name: villin-like
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 96-100 kDa
• GenBank Accession Number: BC000243
• Gene Symbol: VILL
• Gene ID (NCBI): 50853
• RRID: AB_2918038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15195
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.