TrpRS Polyclonal antibody

• Featured Product
• KD/KO Validated

TrpRS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16081-1-AP

Synonyms

WARS, Interferon-induced protein 53, IFP53, IFI53, hWRS

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Validation Data Gallery

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  WB analysis using 16081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16081-1-AP

  IP result of anti-TrpRS (IP:16081-1-AP, 4ug; Detection:16081-1-AP 1:500) with HepG2 cells lysate 3000ug.

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  IHC staining of human ovary tumor using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16081-1-AP

  Immunofluorescent analysis of HepG2 cells, using WARS antibody 16081-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 16081-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using TrpRS antibody (16081-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 16081-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TrpRS Polyclonal antibody (16081-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, HepG2 cells, Jurkat cells, JAR cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human ovary tumor tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16081-1-AP targets TrpRS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TrpRS fusion protein Ag9079
• Full Name: tryptophanyl-tRNA synthetase
• Calculated Molecular Weight: 471 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC017489
• Gene Symbol: WARS1
• Gene ID (NCBI): 7453
• RRID: AB_2273061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TrpRS is also named as IFI53, WRS, WARS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned (PMID:7743129). It can exsit as a dimer(PMID:7743129). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	J Immunother Cancer; Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation.; Authors - Rui Qin ; KO Validated
human	IHC	Heliyon; Diagnostic, prognostic, and immunological roles of CDSN in ovarian cancer; Authors - Huan Zeng
human	WB	Cell Mol Life Sci; Tryptophanylation of insulin receptor by WARS attenuates insulin signaling; Authors - Wen-Xing Sun ; KD Validated