WASF4-Specific Polyclonal antibody

WASF4-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19755-1-AP

Synonyms

SCAR2, WASF4, WAS protein family, member 4

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Validation Data Gallery

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  WB analysis of mouse brain using 19755-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19755-1-AP (WASF4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19755-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19755-1-AP (WASF4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 19755-1-AP

  Immunofluorescent analysis of HepG2 cells, using WASF4 antibody 19755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19755-1-AP targets WASF4-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: WAS protein family, member 4
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: Q8IV90
• Gene Symbol: WASF4
• Gene ID (NCBI): 644739
• RRID: AB_10646483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WASF4, also named as SCAR2, belongs to the SCAR/WAVE family. WASF4 is a downstream effector molecule which involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. The antibody is specific to WASF4.