WBP11 Polyclonal antibody
WBP11 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10316-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse heart tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
10316-1-AP targets WBP11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | WBP11 fusion protein Ag0366 |
Full Name | WW domain binding protein 11 |
Calculated Molecular Weight | 70 kDa |
Observed Molecular Weight | 66-70 kDa |
GenBank Accession Number | BC001621 |
Gene Symbol | WBP11 |
Gene ID (NCBI) | 51729 |
RRID | AB_10694664 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WBP11 (WW domain-binding protein 11) is a protein that in humans, is encoded by the WBP11 gene. The function of WBP11 is to play a role in the regulation of pre-mRNA processing. More specifically, this nuclear protein, colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. The predicted MW of this protein is 70 kDa, and this antibody specially recognises the 70 kDa protein.
Protocols
Product Specific Protocols | |
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WB protocol for WBP11 antibody 10316-1-AP | Download protocol |
IHC protocol for WBP11 antibody 10316-1-AP | Download protocol |
IF protocol for WBP11 antibody 10316-1-AP | Download protocol |
IP protocol for WBP11 antibody 10316-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |