WDR12 Polyclonal antibody

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• WDR12 Polyclonal antibody (33831-1-AP)

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  WB analysis using 33831-1-AP

  HeLa cells lysates were subjected to SDS PAGE followed by western blot with 33831-1-AP (WDR12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

WDR12 Polyclonal Antibody for WB, ELISA

Cat No. 33831-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Ribosome biogenesis protein WDR12, WD repeat-containing protein 12

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33831-1-AP targets WDR12 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38016

  Product name: Recombinant human WDR12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 74-423 aa of BC008082

  Sequence: NISSEEVVEIEYVEKYTAPQPEQCMFHDDWISSIKGAEEWILTGSYDKTSRIWSLEGKSIMTIVGHTDVVKDVAWVKKDSLSCLLLSASMDQTILLWEWNVERNKVKALHCCRGHAGSVDSIAVDGSGTKFCSGSWDKMLKIWSTVPTDEEDEMEESTNRPRKKQKTEQLGLTRTPIVTLSGHMEAVSSVLWSDAEEICSASWDHTIRVWDVESGSLKSTLTGNKVFNCISYSPLCKRLASGSTDRHIRLWDPRTKDGSLVSLSLTSHTGWVTSVKWSPTHEQQLISGSLDNIVKLWDTRSCKAPLYDLAAHEDKVLSVDWTDTGLLLSGGADNKLYSYRYSPTTSHVGA

• Full Name: WD repeat domain 12
• Calculated Molecular Weight: 423 aa, 48 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC008082
• Gene Symbol: WDR12
• Gene ID (NCBI): 55759
• RRID: AB_3743085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9GZL7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR12 is a nucleolar WD-repeat scaffolding protein and an essential component of the PeBoW complex required for 60S ribosomal subunit maturation. By driving pre-rRNA processing, WDR12 supports cell growth and proliferation, while its disruption activates the p53-dependent nucleolar stress pathway. Upregulated in multiple cancers and genetically linked to cardiometabolic traits, WDR12 serves as a central regulator of ribosome biogenesis, cell-cycle control, and cellular homeostasis.