WDR21C Recombinant monoclonal antibody

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  WB analysis using 83802-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83802-4-RR (WDR21C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83802-4-RR

  WB result of WDR21C antibody (83802-4-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR21C transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 83802-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83802-4-RR against Human WDR21C were performed. The affinity constant is 0.479 nM.

• Featured Product
• KD/KO Validated

WDR21C Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83802-4-RR

Clone No.240402H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

240402H3, DCAF4L2, DDB1- and CUL4-associated factor 4-like protein 2, WD repeat domain 21C, WD repeat-containing protein 21C

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83802-4-RR targets WDR21C in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30748

  Product name: Recombinant human WDR21C protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 46-395 aa of BC111778

  Sequence: ARELRVSCMQRKKVQIHSWDPSSLASDRFNRILANTNTDQLFTVNQVEAGGSKYGIITMRGLTTPELRVYPHKTLYVPNRKVNSMCWASLNHLDSHLLLCFVGLADTPSCAVLLPASLFIGSFPGMRRPGMLCSFQIPDAWSCAWSLSIHAYHSFSTGLSQQVLLTNVVTGHQQSFGTSSDVLAQQFAIMTPLLFNGCRSGEIFGIDLRCGNQGSGWKAICLSHDSAVTSLQILQDGQFLVSSDMTGTIKLWDLRATKCVTQYEGHVNNSAYLPVHVNEEEGVVAAVGQDCYTRIWSLRHGHLLTTIPSPYPASENDIPSVAFSSRLGGFRGAPGLLMAVREDLYCFSYG

• Full Name: WD repeat domain 21C
• Calculated Molecular Weight: 395 aa, 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC111778
• Gene Symbol: WDR21C
• Gene ID (NCBI): 138009
• RRID: AB_3671390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NA75
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR21C (WD repeat-containing protein 21C), also known as DCAF4L2. It is expected to be located in the cytoplasm, which is enriched in testis. WDR21C is a member of WD-repeat proteins, which commonly serve as mediators of protein-protein interplay. It is reported that elevated WDR21C expression in human colorectal cancer (CRC) significantly correlated with a more advanced clinical stage as in lymphatic and distant metastasis. More importantly, elevated WDR21C expression is an independent prognosis factor for survival (PMID: 27158335). The molecular weight of WDR21C is 43 kDa.