WDR48 Polyclonal antibody

• Featured Product
• KD/KO Validated

WDR48 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 16503-1-AP

Synonyms

WD repeat domain 48, USP1-associated factor 1, USP1 associated factor 1, UAF1, p80

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Validation Data Gallery

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  WB analysis using 16503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16503-1-AP (WDR48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16503-1-AP

  IP result of anti-WDR48 (IP:16503-1-AP, 3ug; Detection:16503-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 16503-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16503-1-AP (WDR48 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, mouse heart tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IP: See 1 publications below

Product Information

16503-1-AP targets WDR48 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR48 fusion protein Ag9674
• Full Name: WD repeat domain 48
• Calculated Molecular Weight: 502aa,57 kDa; 677aa,76 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC037168
• Gene Symbol: WDR48
• Gene ID (NCBI): 57599
• RRID: AB_2878266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IP	Nat Commun; The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling; Authors - Victoria H Ng
human	WB	Cell Prolif; Comprehensive analysis of ubiquitin-specific protease 1 reveals its importance in hepatocellular carcinoma.; Authors - Yalei Zhao
mouse	WB	J Neurosci; USP1/UAF1-Stabilized METTL3 Promotes Reactive Astrogliosis and Improves Functional Recovery after Spinal Cord Injury through m6A Modification of YAP1 mRNA; Authors - Xuhui Ge ; KD Validated
human	WB	Cell Cycle; USP1-WDR48 deubiquitinase complex enhances TGF-β induced epithelial-mesenchymal transition of TNBC cells via stabilizing TAK1.; Authors - Dianwen Han ; KD Validated
human	WB	J Biol Chem; The WD40-repeat protein WDR-48 promotes the stability of the deubiquitinating enzyme USP-46 by inhibiting its ubiquitination and degradation.; Authors - Molly Hodul ; KD Validated
human	WB	Microbiol Spectr; USP1-Associated Factor 1 Modulates Japanese Encephalitis Virus Replication by Governing Autophagy and Interferon-Stimulated Genes; Authors - Jinchao Xing ; KD Validated