WDR76 Polyclonal antibody

WDR76 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, RIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25528-1-AP

Synonyms

CDW14, WD repeat domain 76, WDR76

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Validation Data Gallery

• 

  WB analysis of HeLa using 25528-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25528-1-AP (WDR76 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

25528-1-AP targets WDR76 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR76 fusion protein Ag22114
• Full Name: WD repeat domain 76
• Calculated Molecular Weight: 626 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC051855
• Gene Symbol: WDR76
• Gene ID (NCBI): 79968
• RRID: AB_2880120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WD40 repeat protein 76 (WDR76) is identified as a novel tumor suppressor and RAS activity can be controlled via regulating protein stability which further affect the tumorigenesis and metastasis in different cancer diseases.

Publications

Species	Application	Title
human	WB,IHC	Clin Transl Med; LRPPRC promotes glycolysis by stabilising LDHA mRNA and its knockdown plus glutamine inhibitor induces synthetic lethality via m6 A modification in triple-negative breast cancer; Authors - Yuanhang Yu
human	WB,RIP,IF	Cell Prolif; CircSMAD3 represses VSMC phenotype switching and neointima formation via promoting hnRNPA1 ubiquitination degradation; Authors - Shuai Mei