WEE2 Polyclonal antibody

WEE2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55119-1-AP

Synonyms

WEE1 homolog 2 (S. pombe), Wee1 like protein kinase 1B, Wee1 like protein kinase 2, WEE1B, Wee1B kinase, WEE2

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Validation Data Gallery

• 

  WB analysis of Jurkat using 55119-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55119-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ovary using 55119-1-AP

  human ovary tissue were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, human ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55119-1-AP targets WEE2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: WEE1 homolog 2 (S. pombe)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63-66 kDa
• GenBank Accession Number: NM_001105558
• Gene Symbol: WEE2
• Gene ID (NCBI): 494551
• RRID: AB_10951857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WEE2, also named as WEE1B, belongs to the protein kinase superfamily. Ser/Thr protein kinase family and WEE1 subfamily. It phosphorylates and inhibits CDK1. WEE2 may act as a negative regulator of entry into mitosis (G2 to M transition). WEE2 is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Taking the long double-stranded RNA-mediated RNA interference approach, down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. Overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.(PMID: 20200212) The antibody is specific to WEE2.