Filter: AllWBIHCIF/ICC

WB analysis using 67210-1-Ig (same clone as 67210-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67210-1-Ig (WNT10B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 67210-1-Ig (same clone as 67210-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 67210-1-Ig (same clone as 67210-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 67210-1-Ig (same clone as 67210-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 67210-1-Ig (same clone as 67210-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 67210-1-Ig (same clone as 67210-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 67210-1-Ig (same clone as 67210-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67210-1-Ig (same clone as 67210-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67210-1-Ig (WNT10B antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

WNT10B Monoclonal antibody, PBS Only

WNT10B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67210-1-PBS

Clone No.1G11E4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1G11E4, Protein Wnt 10b, Protein Wnt 12, Protein Wnt-10b, Protein Wnt-12

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67210-1-PBS targets WNT10B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag17114 Product name: Recombinant human WNT10B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-204 aa of BC096353 Sequence: FLALCSRALSNEILGLKLPGEPPLTANTVCLTLSGLSKRQLGLCLRNPDVTASALQGLHIAVHECQHQLRDQRWNCSALEGGGRLPHHSAILKRGFRESAFSFSMLAAGVMHAVATACSLGKLVSCGCGWKGSGEQDRLRAKLLQLQALSRGKSFPHSLPSPGPGSSPSPGPQDTWEWGGCNHDMD Predict reactive species
Full Name	wingless-type MMTV integration site family, member 10B
Calculated Molecular Weight	389 aa, 43 kDa
Observed Molecular Weight	43 kDa
GenBank Accession Number	BC096353
Gene Symbol	WNT10B
Gene ID (NCBI)	7480
RRID	AB_2882503
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O00744
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.