WNT3 Polyclonal antibody

WNT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse





Cat no : 28156-1-AP


INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, WNT3

Tested Applications

Positive WB detected inHCT 116 cells, MCF-7 cells
Positive IHC detected inmouse colon tissue, mouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28156-1-AP targets WNT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT3 fusion protein Ag27965
Full Name wingless-type MMTV integration site family, member 3
Calculated Molecular Weight 355 aa, 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC114219
Gene Symbol WNT3
Gene ID (NCBI) 7473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WNT3 is also named as INT4. WNT3 is required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PMID: 14872406). Wnt3 is up-regulated in cancer tissues, such as gastric cancer and lung cancer. Wnt3, as a member of Wnt family, has been proved to promote the stabilization of β-catenin to regulate the radiation sensitivity of cancer cells (PMID: 31851619).


Product Specific Protocols
WB protocol for WNT3 antibody 28156-1-APDownload protocol
IHC protocol for WNT3 antibody 28156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols