WNT3 Polyclonal antibody, PBS Only

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  WB analysis using 28156-1-AP (same clone as 28156-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28156-1-AP (WNT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28156-1-AP (same clone as 28156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 28156-1-AP (same clone as 28156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

WNT3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28156-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3

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Product Information

28156-1-PBS targets WNT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27965

  Product name: Recombinant human WNT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 130-306 aa of BC114219

  Sequence: SCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHG

• Full Name: wingless-type MMTV integration site family, member 3
• Calculated Molecular Weight: 355 aa, 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC114219
• Gene Symbol: WNT3
• Gene ID (NCBI): 7473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56703
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

WNT3 is also named as INT4. WNT3 is required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PMID: 14872406). Wnt3 is up-regulated in cancer tissues, such as gastric cancer and lung cancer. Wnt3, as a member of Wnt family, has been proved to promote the stabilization of β-catenin to regulate the radiation sensitivity of cancer cells (PMID: 31851619).