WNT3 Monoclonal antibody

• 

  WB analysis using 67452-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67452-1-Ig (WNT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

WNT3 Monoclonal Antibody for WB, ELISA

Cat No. 67452-1-Ig

Clone No.2B4D11

Publications(4)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, ELISA

Synonyms

INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, WNT3

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Tested Applications

• Positive WB detected in: pig brain tissue, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

67452-1-Ig targets WNT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27957

  Product name: Recombinant human WNT3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 130-306 aa of BC114219

  Sequence: SCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHG

• Full Name: wingless-type MMTV integration site family, member 3
• Calculated Molecular Weight: 355 aa, 40 kDa
• GenBank Accession Number: BC114219
• Gene Symbol: WNT3
• Gene ID (NCBI): 7473
• RRID: AB_2882686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P56703
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Cell Int; miR-454 suppresses the proliferation and invasion of ovarian cancer by targeting E2F6.; Authors - Yunhe An
human	IHC, WB	Chin J Integr Med; Babao Dan () Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway.; Authors - Meng-Xuan Gui
mouse	WB,IHC	Kidney Int; Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis; Authors - Dong Zhou
human		World J Gastrointest Oncol; N-glycosylation of Wnt3 regulates the progression of hepatocellular carcinoma by affecting Wnt/β-catenin signal pathway; Authors - Xin-Zhan Zhang