WSB1 Polyclonal antibody

WSB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11666-1-AP

Synonyms

WSB-1, WSB 1, WD repeat and SOCS box-containing protein 1, SWIP1, SOCS box-containing WD protein SWiP-1

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Validation Data Gallery

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  WB analysis using 11666-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11666-1-AP (WSB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11666-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11666-1-AP (WSB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11666-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WSB1 antibody (11666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells, SMMC-7721 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

11666-1-AP targets WSB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WSB1 fusion protein Ag2211
• Full Name: WD repeat and SOCS box-containing 1
• Calculated Molecular Weight: 421 aa, 47 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC021110
• Gene Symbol: WSB1
• Gene ID (NCBI): 26118
• RRID: AB_10949076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WSB1 (or SWIP1) gene encodes WD repeat and SOCS box-containing protein 1, a member of the WD-protein subfamily. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding 3 distinct isoforms have been found for this gene. WSB1 serves as a probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WSB1 has been reported (PMID: 15965468), initiated by the interaction of the two components (PMID: 18093972). WSB1 is also a part of an E3 ubiquitin ligase for the thyroid-hormone-activating type 2 iodothyronine deiodinase (D2) (PMID: 15965468).

Publications

Species	Application	Title
human	WB	Cell Res; WSB1 overcomes oncogene-induced senescence by targeting ATM for degradation.; Authors - Jung Jin Kim
human	WB	Genes Dev; WSB1 promotes tumor metastasis by inducing pVHL degradation.; Authors - Jung Jin Kim
human	WB	Cancer Res; Hypoxia-Induced WSB1 Promotes the Metastatic Potential of Osteosarcoma Cells.; Authors - Ji Cao
human,mouse	IHC	Front Immunol; Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression; Authors - Zhao Guang
human	WB	Toxicology; BDE209-promoted Dio2 degradation in H4 glioma cells through the autophagy pathway, resulting in hypothyroidism and leading to neurotoxicity; Authors - Min Liu
human	WB,IHC	Int J Biol Macromol; circWSB1 promotes tumor progression in ccRCC via circWSB1/miR-182-5p/WSB1 axis; Authors - Guyu Tang