WSB2 Polyclonal antibody

WSB2 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12124-2-AP

Synonyms

CS box containing WD protein, SBA2, WSB 2, WSB2

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 12124-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12124-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12124-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 12124-2-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12124-2-AP

  IP result of anti-WSB2 (IP:12124-2-AP, 3ug; Detection:12124-2-AP 1:800) with mouse kidney tissue lysate 6000ug.

• 

  IF Staining of HepG2 using 12124-2-AP

  Immunofluorescent analysis of HepG2 cells, using WSB2 antibody 12124-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: PC-3 cells, rat kidney tissue, mouse kidney tissue, human brain tissue
• Positive IP detected in: mouse kidney tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12124-2-AP targets WSB2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WSB2 fusion protein Ag2768
• Full Name: WD repeat and SOCS box-containing 2
• Calculated Molecular Weight: 404 aa, 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC015887
• Gene Symbol: WSB2
• Gene ID (NCBI): 55884
• RRID: AB_2216206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WSB2, also named SBA2, encodes WD repeat and SOCS box-containing protein 2. WSB2 may be a substrate-recognition component of an E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. WSB2 is a novel protein supposed to be involved in male- and female-specific gonadal development, and also a regulator of IL-21 receptor expression and IL21-induced signal transduction. WSB2 might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological functions need further studies.

Publications

Species	Application	Title
human	IHC	Cell Rep; Prognostic impact of vitamin B6 metabolism in lung cancer.; Authors - Galluzzi Lorenzo L
human	IP	Elife; Multiple cullin-associated E3 ligases regulate cyclin D1 protein stability; Authors - Ke Lu
	IHC	Oncoimmunology; Metabolic enzymes expressed by cancer cells impact the immune infiltrate.; Authors - Gautier Stoll
human	WB,IHC,CoIP	J Oncol; Retinoblastoma-Binding Protein 5 Regulates H3K4 Methylation Modification to Inhibit the Proliferation of Melanoma Cells by Inactivating the Wnt/β-Catenin and Epithelial-Mesenchymal Transition Pathways; Authors - Zhiqin Yang ; KD Validated