WWTR1/TAZ Polyclonal antibody

• Featured Product
• KD/KO Validated

WWTR1/TAZ Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

56

Reviews (2)

Cat no : 23306-1-AP

Synonyms

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif

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Validation Data Gallery

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  WB analysis using 23306-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23306-1-AP (TAZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23306-1-AP

  WB result of TAZ antibody (23306-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HeLa cells.

• 

  WB analysis of LN229 cells using 23306-1-AP

  WB result of WWTR1 antibody (TAZ antibody, 23306-1-AP, 1:1000) with LN229 cell lysate by Dr. Xiaobing He. (Lane 1, LN229 (GBM cell line) cell lysate. 90 ug; Lane 2, LN229 growth inhibition treatment. 90 ug).

• 

  WB analysis of MDA-MB-453s using 23306-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 23306-1-AP (WWTR1,TAZ Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MDA-MB-453s using 23306-1-AP

  IP result of anti-WWTR1 (IP:23306-1-AP, 4ug; Detection:23306-1-AP 1:300) with MDA-MB-453s cells lysate 2800ug.

• 

  IP experiment of MDA-MB-453s using 23306-1-AP

  IP result of anti-WWTR1 (IP:23306-1-AP, 4ug; Detection:23306-1-AP 1:5000) with MDA-MB-453s cells lysate 2800 ug.

• 

  IHC staining of human ovary cancer using 23306-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23306-1-AP (WWTR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat kidney using 23306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat kidney tissue using WWTR1 antibody (23306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U-251 using 23306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using WWTR1 antibody (23306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, LN229 cells, MDA-MB-453s cells
• Positive IP detected in: MDA-MB-453s cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat kidney tissue
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 47 publications below
• IHC: See 11 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

23306-1-AP targets WWTR1/TAZ in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WWTR1/TAZ fusion protein Ag19659
• Full Name: WW domain containing transcription regulator 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC014052
• Gene Symbol: WWTR1
• Gene ID (NCBI): 25937
• RRID: AB_2721185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma (PMID: 22003437, PMID: 27764783). TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Publications

Species	Application	Title
human,mouse	IF,IP	Cancer Cell; Programming of Schwann Cells by Lats1/2-TAZ/YAP Signaling Drives Malignant Peripheral Nerve Sheath Tumorigenesis.; Authors - Lai Man Natalie Wu
human	WB,IHC	Nat Neurosci; YAP and TAZ control peripheral myelination and the expression of laminin receptors in Schwann cells.; Authors - Yannick Poitelon ; KO Validated
human	WB	Adv Sci (Weinh); DNMT3A Cooperates with YAP/TAZ to Drive Gallbladder Cancer Metastasis; Authors - Sunwang Xu
mouse,human	WB	Sci Adv; YAP drives fate conversion and chemoresistance of small cell lung cancer.; Authors - Qingzhe Wu ; KD Validated
mouse	WB,IF	Bone Res; TAZ inhibits osteoclastogenesis by attenuating TAK1/NF-κB signaling.; Authors - Wanlei Yang ; KO Validated
human	WB	Clin Transl Med; NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization; Authors - Shaomin Zou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yohanes (Verified Customer) (04-24-2023)

• Applications: Western Blot, Immunofluorescence, Other
• Primary Antibody Dilution: 1:1000 for WB, 1:200 for IF, 1:100 for PLA
• Cell Tissue Type: bEnd.3 mouse Endothelial cell

FH

Joshua (Verified Customer) (02-28-2020)

MDCKs fixed in 4% paraformaldehyde. Bright Mix of nuclear and cytosolic staining

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDCK

WWTR1/TAZ Antibody Immunofluorescence, validation (1:500 dilution) in MDCK (Cat no:23306-1-AP)

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