• Overview
  • Tested Applications
  • Product Information
  • Publications (8)
  • Protocols
  • Featured Product
  • KD/KO Validated

XPO6 Polyclonal antibody

XPO6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 11408-1-AP

Synonyms

EXP6, exportin 6, KIAA0370, Ran binding protein 20, RANBP20, XPO6



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11408-1-AP targets XPO6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XPO6 fusion protein Ag1898
Full Name exportin 6
Calculated Molecular Weight 1125 aa, 129 kDa
Observed Molecular Weight 128 kDa
GenBank Accession NumberBC014071
Gene Symbol XPO6
Gene ID (NCBI) 23214
RRIDAB_2241610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human XPO6 gene encodes a Ran-binding protein 20, exportin-6, belonging to the exportin family. Exportin-6 shuttles between the nucleus and the cytoplasm and mediates the nuclear export of actin and profilin-actin complexes in somatic cells. Interaction of exportin-6 and actin is achieved in a RanGTP-dependent manner, actin is a major cytoskeletal element which is normally kept cytoplasmic by exportin-6 driven nuclear export.

Protocols

Product Specific Protocols
WB protocol for XPO6 antibody 11408-1-APDownload protocol
IHC protocol for XPO6 antibody 11408-1-APDownload protocol
IP protocol for XPO6 antibody 11408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanWB

Nat Commun

Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.

Authors - Sergi Marco
DrosophilaIF

Proc Natl Acad Sci U S A

Active maintenance of nuclear actin by importin 9 supports transcription.

Authors - Dopie Joseph J
humanWB

Cancer Res

Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer.

Authors - Ambs Stefan S
humanWB

J Virol

The XPO6 Exportin Mediates Herpes Simplex Virus 1 gM Nuclear Release Late in Infection.

Authors - Hugo Boruchowicz
  • KD Validated
humanWB

RNA

An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.

Authors - Ewan K S McRae
humanWB

JCI Insight

Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis.

Authors - Maryam Aftabizadeh