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XPO6 Polyclonal antibody
XPO6 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 11408-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 7 publications below |
IF | See 2 publications below |
Product Information
11408-1-AP targets XPO6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, drosophila |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | XPO6 fusion protein Ag1898 |
Full Name | exportin 6 |
Calculated Molecular Weight | 1125 aa, 129 kDa |
Observed Molecular Weight | 128 kDa |
GenBank Accession Number | BC014071 |
Gene Symbol | XPO6 |
Gene ID (NCBI) | 23214 |
RRID | AB_2241610 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human XPO6 gene encodes a Ran-binding protein 20, exportin-6, belonging to the exportin family. Exportin-6 shuttles between the nucleus and the cytoplasm and mediates the nuclear export of actin and profilin-actin complexes in somatic cells. Interaction of exportin-6 and actin is achieved in a RanGTP-dependent manner, actin is a major cytoskeletal element which is normally kept cytoplasmic by exportin-6 driven nuclear export.
Protocols
Product Specific Protocols | |
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WB protocol for XPO6 antibody 11408-1-AP | Download protocol |
IHC protocol for XPO6 antibody 11408-1-AP | Download protocol |
IP protocol for XPO6 antibody 11408-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion. | ||
Proc Natl Acad Sci U S A Active maintenance of nuclear actin by importin 9 supports transcription. | ||
Cancer Res Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer. | ||
J Virol The XPO6 Exportin Mediates Herpes Simplex Virus 1 gM Nuclear Release Late in Infection.
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