XPO6 Polyclonal antibody

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• XPO6 Polyclonal antibody (11408-1-AP)

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• KD/KO Validated

XPO6 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11408-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Exp6, exportin 6, exportin6, Exportin-6, KIAA0370

• 

  WB analysis of HeLa using 11408-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11408-1-AP (XPO6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 11408-1-AP

  IP result of anti-XPO6 (IP:11408-1-AP, 4ug; Detection:11408-1-AP 1:300) with HeLa cells lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11408-1-AP (XPO6 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11408-1-AP (XPO6 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11408-1-AP targets XPO6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1898

  Product name: Recombinant human XPO6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 539-888 aa of BC014071

  Sequence: MPLDDTKLIIHQTLSVLEDIVENISGESTKSRQICYQSLQESVQVSLALFPAFIHQSDVTDEMLSFFLTLFRGLRVQMGVPFTEQIIQTFLNMFTREQLAESILHEGSTGCRVVEKFLKILQVVVQEPGQVFKPFLPSIIALCMEQVYPIIAERPSPDVKAELFELLFRTLHHNWRYFFKSTVLASVQRGIAEEQMENEPQFSAIMQAFGQSFLQPDIHLFKQNLFYLETLNTKQKLYHKKIFRTAMLFQFVNVLLQVLVHKSHDLLQEEIGIAIYNMASVDFDGFFAAFLPEFLTSCDGVDANQKSVLGRNFKMDRDLPSFTQNVHRLVNDLRYYRLCNDSLPPGTVKL

• Full Name: exportin 6
• Calculated Molecular Weight: 1125 aa, 129 kDa
• Observed Molecular Weight: 128 kDa
• GenBank Accession Number: BC014071
• Gene Symbol: XPO6
• Gene ID (NCBI): 23214
• RRID: AB_2241610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96QU8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human XPO6 gene encodes a Ran-binding protein 20, exportin-6, belonging to the exportin family. Exportin-6 shuttles between the nucleus and the cytoplasm and mediates the nuclear export of actin and profilin-actin complexes in somatic cells. Interaction of exportin-6 and actin is achieved in a RanGTP-dependent manner, actin is a major cytoskeletal element which is normally kept cytoplasmic by exportin-6 driven nuclear export.

Publications

What published studies show

XPO6 Polyclonal antibody (11408-1-AP) has 9 citations published in Cancer Research, Nature Communications, PNAS, Frontiers in Immunology, JCI Insight, International Immunopharmacology, RNA, Journal of Virology, and Virus Research. Research fields include prostate cancer, nuclear actin dynamics and cancer invasion, transcription regulation, breast cancer immunotherapy, STAT3-mediated antitumor effects, COPD/TLR2 signaling, RNA quadruplex pathways, and viral replication.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Res; Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer.; Authors - Ambs Stefan S
human	WB	Nat Commun; Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.; Authors - Sergi Marco
Drosophila	IF	Proc Natl Acad Sci U S A; Active maintenance of nuclear actin by importin 9 supports transcription.; Authors - Dopie Joseph J
	WB	Front Immunol; An immunotherapy guide constructed by cGAS-STING signature for breast cancer and the biofunction validation of the pivotal gene HOXC13 via in vitro experiments; Authors - Zhenling Dai
human	WB	JCI Insight; Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis.; Authors - Maryam Aftabizadeh
mouse	WB	Int Immunopharmacol; Exportin XPO6 upregulation activates the TLR2/MyD88/NF-κB signaling by facilitating TLR2 mRNA nuclear export in COPD pulmonary monocytes; Authors - Yuting Wu ; KD Validated