XRCC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

XRCC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 21468-1-AP

Synonyms

X-ray repair cross-complementing protein 1, DNA repair protein XRCC1

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Validation Data Gallery

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  WB analysis using 21468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21468-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21468-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21468-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21468-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21468-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21468-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21468-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 21468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21468-1-AP (XRCC1 antibody) at dilution of 1:1000 (under 40x lens).

• 

  IF Staining of HeLa using 21468-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 21468-1-AP (XRCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below

Product Information

21468-1-AP targets XRCC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XRCC1 fusion protein Ag15172
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 1
• Calculated Molecular Weight: 633 aa, 70 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC023593
• Gene Symbol: XRCC1
• Gene ID (NCBI): 7515
• RRID: AB_2878865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XRCC1 (X-ray Repair Cross Complementing group 1) is a molecular scaffold protein that interacts with multiple enzymatic components of DNA single-strand break repair (SSBR) including DNA kinase, DNA phosphatase, DNA polymerase, DNA deadenylase, and DNA ligase activities that collectively are capable of accelerating the repair of a broad range of DNA single-strand breaks (SSBs) (PMID: 18515191). XRCC1 is 633 amino acids in length which is comprised of an N-terminal domain (NTD) of ˜160 amino acids that interacts with Pol β, a central BRCT domain of ˜90 amino acids that interacts with PARP1 (PMID: 9584196).

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
human	WB	Mol Ther; PMID: 29456019; Authors - Xinyi Wang
human	WB	J Cell Mol Med; Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.; Authors - Xingjie Gao
human	WB	Int J Environ Res Public Health; Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM2.5 from Beijing, China, in Winter.; Authors - Bing-Yu Niu
human	WB	Toxicol Appl Pharmacol; Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.; Authors - Hui Dong
human	WB,IHC	Front Mol Biosci; XRCC1 Is a Promising Predictive Biomarker and Facilitates Chemo-Resistance in Gallbladder Cancer.; Authors - Zhengchun Wu ; KD Validated