XYLB Polyclonal antibody

XYLB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26541-1-AP

Synonyms

XYLB, Xylulokinase, Xylulose kinase

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Validation Data Gallery

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  WB analysis of A431 using 26541-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 26541-1-AP (XYLB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 26541-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 26541-1-AP (XYLB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 26541-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 26541-1-AP (XYLB Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26541-1-AP is XYLB Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XYLB fusion protein Ag19208
• Full Name: xylulokinase homolog (H. influenzae)
• Calculated Molecular Weight: 536 aa, 58 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC137076
• Gene Symbol: XYLB
• Gene ID (NCBI): 9942
• RRID: AB_2880546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human xylulokinase-like protein (XYLB), is a 58 kDa protein belongs to a family of enzymes that include fucokinase, gluconokinase, glycerokinase and xylulokinase. XYLB is a key regulator for controlling metabolic disease.XYLB also plays important roles in energy metabolism. XYLB has 2 isoforms produced by alternative splicing with the MW of 58 and 43 kDa.