XYLT1 Polyclonal antibody, PBS Only

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• XYLT1 Polyclonal antibody, PBS Only (55061-1-PBS)

• 

  WB analysis of HEK-293 using 55061-1-AP (same clone as 55061-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55061-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 55061-1-AP (same clone as 55061-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55061-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 55061-1-AP (same clone as 55061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55061-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 55061-1-AP (same clone as 55061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55061-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 55061-1-AP (same clone as 55061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55061-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 55061-1-AP (same clone as 55061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55061-1-PBS in a different storage buffer formulation.

XYLT1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55061-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

55061-1-PBS targets XYLT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: xylosyltransferase I
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_022166
• Gene Symbol: XYLT1
• Gene ID (NCBI): 64131
• RRID: AB_10804758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86Y38
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

XYLT1, also named as XT1, Belongs to the glycosyltransferase 14 family and XylT subfamily. It catalyzes the first step in biosynthesis of glycosaminoglycan. XYLT1 transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. The antibody is specific to XYLT1.