Phospho-YAP1 (Ser127) Recombinant antibody, PBS Only

• 

  WB analysis of HepG2 using 80694-2-RR (same clone as 80694-2-PBS)

  Calyculin A treated and untreated HepG2 cells were subjected to SDS PAGE followed by western blot with 80694-2-RR (Phospho-YAP1 (Ser127) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 80694-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 80694-2-RR (same clone as 80694-2-PBS)

  1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A intracellularly stained with 0.13 ug Phospho-YAP1 (Ser127) Recombinant antibody (80694-2-RR, Clone:240779G4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80694-2-PBS in a different storage buffer formulation.

YAP1 antibodies are used to detect Yes-associated protein 1, a critical effector in the Hippo pathway, in studies of cell proliferation, organ size regulation, and cancer. Proteintech has an extensive validated YAP1 antibodies for WB, FC (Intra), ELISA.

Cat No. 80694-2-PBS

Clone No.240779G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

p YAP1, p YAP1 (Ser127), p YAP1 Ser127, Phospho YAP1, Phospho YAP1 (Ser127)

---

Product Information

80694-2-PBS targets Phospho-YAP1 (Ser127) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: Yes-associated protein 1, 65kDa
• Calculated Molecular Weight: 504 aa, 54 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC038235
• Gene Symbol: YAP1
• Gene ID (NCBI): 10413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P46937
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)