MSY2 Polyclonal antibody

MSY2 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13538-1-AP

Synonyms

Germ cell-specific Y-box-binding protein, DNA-binding protein C, DNA binding protein C, Dbpc, CSDA3

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Validation Data Gallery

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  WB analysis of human testis using 13538-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13538-1-AP (MSY2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 13538-1-AP

  IP result of anti-MSY2 (IP:13538-1-AP, 4ug; Detection:13538-1-AP 1:300) with PC-3 cells lysate 1720ug.

• 

  IHC staining of human ovary tumor using 13538-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13538-1-AP (MSY2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 13538-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13538-1-AP (MSY2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Product Information

13538-1-AP targets MSY2 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSY2 fusion protein Ag4465
• Full Name: Y box binding protein 2
• Calculated Molecular Weight: 364 aa, 39 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC033800
• Gene Symbol: YBX2
• Gene ID (NCBI): 51087
• RRID: AB_2241777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSY2, also named CSDA3 and YBX2, belongs to the Y box family of cold shock domain proteins. MSY2 is solely expressed in male and female germ cells and is necessary for fertility. MSY2 serves as a coactivator of transcription, binding to the DNA sequence CTGATTGGC/TC/TAA in the promoters of genes transcribed in germ cells, thereby linking transcription with mRNA storage/stabilization (PMID: 19597149). MSY2 has 1 isoform with a molecular mass of 38 kDa. MSY2 is essential for regulating mRNA stability in oocytes (PMID: 21613634).

Publications

Species	Application	Title
mouse	WB,IHC,chIP	J Clin Invest; Methylation in pericytes after acute injury promotes chronic kidney disease.; Authors - Yu-Hsiang Chou
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang