MSY2 Polyclonal antibody

MSY2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 13538-1-AP

Synonyms

CONTRIN, CSDA3, DBPC, DNA binding protein C, MSY2, MSY2 homolog, Y box binding protein 2, YBX2



Tested Applications

Positive WB detected inHeLa cells, human stomach tissue, human testis tissue, HepG2 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13538-1-AP targets MSY2 in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MSY2 fusion protein Ag4465
Full Name Y box binding protein 2
Calculated Molecular Weight 364 aa, 39 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC033800
Gene Symbol YBX2
Gene ID (NCBI) 51087
RRIDAB_2241777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSY2, also named as CSDA3 and YBX2, belongs to the Y box family of cold shock domain proteins. MSY2 is solely expressed in male and female germ cells and necessary for fertility. MSY2 serves as a coactivator of transcription, binding to the DNA sequence CTGATTGGC/TC/TAA in the promoters of genes transcribed in germ cells, thereby linking transcription with mRNA storage/stabilization (PMID: 19597149). MSY2 has 1 isoform with the molecular mass of 38 kDa. MSY2 is essential for regulating mRNA stability in oocytes (PMID: 21613634).

Protocols

Product Specific Protocols
WB protocol for MSY2 antibody 13538-1-APDownload protocol
IHC protocol for MSY2 antibody 13538-1-APDownload protocol
IP protocol for MSY2 antibody 13538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,chIP

J Clin Invest

Methylation in pericytes after acute injury promotes chronic kidney disease.

Authors - Yu-Hsiang Chou
mouseWB,IF,IP

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis

Authors -
mouseWB,IF,IP

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.

Authors - Xiaoli Wang