YIF1B Polyclonal antibody

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  WB analysis using 16406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16406-1-AP (YIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

YIF1B Polyclonal Antibody for WB, ELISA

Cat No. 16406-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

YIP1-interacting factor homolog B, UNQ3073/PRO9905, Protein YIF1B, PP4519, FinGER8

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Tested Applications

• Positive WB detected in: SK-N-SH cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16406-1-AP targets YIF1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YIF1B fusion protein Ag9538
• Full Name: Yip1 interacting factor homolog B (S. cerevisiae)
• Calculated Molecular Weight: 294 aa, 32 kDa
• Observed Molecular Weight: 31~34 kDa
• GenBank Accession Number: BC014974
• Gene Symbol: YIF1B
• Gene ID (NCBI): 90522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5BJH7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YIF1B is an intracellular membrane-bound protein belonging to the Yip family, a trafficking protein orthologue of YIF1P that was initially identified in Saccharomyces cerevisiae as interacting with YIP1P and essential for secretion. YIF1B is expressed in various tissues, especially in brain neurons (PMID: 33103737). YIF1B is localized in the intermediate compartment between the endoplasmic reticulum (ER) and the Golgi apparatus (PMID: 26077767). Western blot analysis detected YIF1B at an apparent molecular mass of 31 kDa.