YIPF4 Polyclonal antibody, PBS Only

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• YIPF4 Polyclonal antibody, PBS Only (15473-1-PBS)

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  WB analysis of HeLa using 15473-1-PBS

  HeLa cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15473-1-AP (same clone as 15473-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 15473-1-PBS

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 15473-1-AP (same clone as 15473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 15473-1-AP (same clone as 15473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15473-1-AP (same clone as 15473-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using YIPF4 antibody (15473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15473-1-AP (same clone as 15473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15473-1-AP (YIPF4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL594-phalloidin staining of F-actin. This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

YIPF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15473-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

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Product Information

15473-1-PBS targets YIPF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7777

  Product name: Recombinant human YIPF4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-113 aa of BC004875

  Sequence: MQPPGPPPAYAPTNGDFTFVSSADAEDLSGSIASPDVKLNLGGDFIKESTATTFLRQRGYGWLLEVEDDDPEDNKPLLEELDIDLKDIYYKIRCVLMPMPSLGFNRQVVRDNP

• Full Name: Yip1 domain family, member 4
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC004875
• Gene Symbol: YIPF4
• Gene ID (NCBI): 84272
• RRID: AB_2217206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BSR8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.