YLPM1 Polyclonal antibody

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• YLPM1 Polyclonal antibody (21332-1-AP)

• 

  WB analysis using 21332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21332-1-AP (YLPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 21332-1-AP

  WB result of YLPM1 antibody (21332-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YLPM1 transfected SH-SY5Y cells.

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  IP experiment of Neuro-2a using 21332-1-AP

  IP result of anti-YLPM1 (IP:21332-1-AP, 4ug; Detection:21332-1-AP 1:1000) with Neuro-2a cells lysate 1440 ug.

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  IF Staining of SH-SY5Y using 21332-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using YLPM1 antibody (21332-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• KD/KO Validated

YLPM1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 21332-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

C14orf170, YLP motif-containing protein 1, YLPM 1, ZAP, ZAP113

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, U-251 cells, Neuro-2a cells, C6 cells
• Positive IP detected in: Neuro-2a cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21332-1-AP targets YLPM1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15408

  Product name: Recombinant human YLPM1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1960-2146 aa of BC023570

  Sequence: MSADNQTCGKRNIHGRKLKEINKMADHWETAPRHMMRLDIRSLLQDAAIEEVEMEDFDANIEEQKEEKKDAEEEESELGYIPKSKWEMDTSEAKLDKLDGLRTGTKRKRDWEAIASRMEDYLQLPDDYDTRASEPGKKRVRWADLEEKKDADRKRAIGFVVGQTDWEKITDESGHLAEKALNRTKYI

• Full Name: YLP motif containing 1
• Calculated Molecular Weight: 1951 aa, 220 kDa
• Observed Molecular Weight: 200 kDa, 250-290 kDa
• GenBank Accession Number: BC023570
• Gene Symbol: YLPM1
• Gene ID (NCBI): 56252
• RRID: AB_3742028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YLPM1 also known as ZAP3, ZAP113, or C14orf170. YLPM1 plays a critical role in the regulation of telomerase activity and transcriptional repression. The molecular weight of the human YLPM1 protein is approximately 250-300 kDa, with a shorter isoform about 200 kDa.