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YME1L1 Monoclonal antibody

YME1L1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7E4

Cat no : 66551-1-Ig

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Sample-dependent, check data in validation data gallery

Product Information

66551-1-Ig targets YME1L1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Reactivity Human, Rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen YME1L1 fusion protein Ag2069
Full Name YME1-like 1 (S. cerevisiae)
Calculated molecular weight 716 aa, 80 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC023507
Gene symbol YME1L1
Gene ID (NCBI) 10730
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

Protocols

Product Specific Protocols
WB protocol for YME1L1 antibody 66551-1-IgDownload protocol
IHC protocol for YME1L1 antibody 66551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols