• Featured Product
  • KD/KO Validated

YTHDC1 Polyclonal antibody

YTHDC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 29441-1-AP

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MDA-MB-231 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29441-1-AP targets YTHDC1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDC1 fusion protein Ag31459
Full Name YTH domain containing 1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight~100 kDa
GenBank Accession NumberBC053863
Gene Symbol YTHDC1
Gene ID (NCBI) 91746
RRIDAB_2918307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

Protocols

Product Specific Protocols
WB protocol for YTHDC1 antibody 29441-1-APDownload protocol
IHC protocol for YTHDC1 antibody 29441-1-APDownload protocol
IP protocol for YTHDC1 antibody 29441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell Int

YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.

Authors - Xiaolong Zhu
  • KD Validated
mouseWB

J Clin Lab Anal

Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.

Authors - Zhenwei Li
humanWB

Nanoscale Res Lett

RNA m6A Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation.

Authors - Jinbin Pan
humanWB

Discov Oncol

Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma

Authors - Fanhua Kong
humanRIP

Cell Stem Cell

Decoding m6A RNA methylome identifies PRMT6-regulated lipid transport promoting AML stem cell maintenance

Authors - Ying Cheng
humanWB,IHC,IP

Exp Mol Med

The YTHDC1/GLUT3/RNF183 axis forms a positive feedback loop that modulates glucose metabolism and bladder cancer progression

Authors - Bin Yan
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zhongwen (Verified Customer) (09-25-2023)

Clear band around 100KD

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
YTHDC1 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:29441-1-AP)