YTHDC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

YTHDC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (2)

Cat no : 27779-1-AP

Synonyms

YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2

---

Validation Data Gallery

• 

  WB analysis using 27779-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27779-1-AP (YTHDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27779-1-AP

  WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HepG2 cells.

• 

  WB analysis of HeLa using 27779-1-AP

  WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HeLa cells.

• 

  IHC staining of rat testis using 27779-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 27779-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27779-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 27779-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using YTHDC2 antibody (27779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 27779-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human YTHDC2 (27779-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
• Positive IHC detected in: rat testis tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

27779-1-AP targets YTHDC2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDC2 fusion protein Ag26690
• Full Name: YTH domain containing 2
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC137285
• Gene Symbol: YTHDC2
• Gene ID (NCBI): 64848
• RRID: AB_2880970
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; Inhibition of METTL3 in macrophages provides protection against intestinal inflammation; Authors - Huilong Yin
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Sci Adv; Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).; Authors - Ji Hoon Lee
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
human	WB,RIP	Cancer Lett; LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation; Authors - Lu Chen ; KD Validated
mouse	WB,IP	Genes Dev; YTHDC2 control of gametogenesis requires helicase activity but not m6A binding.; Authors - Yuhki Saito

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Maialen (Verified Customer) (02-10-2022)

YTHDC2 was immunoprecipitated from 4 million HCT15 cells lysate using 1μg 27779-1-AP (YTHDC2 antibody). Same amount of normal rabbit IgG was used as negative control. For IP confirmation, western blot with 27779-1-AP (YTHDC2 antibody) was perfomed at 1:1000 dilution incubated overnight at 4ºC. 10% of the whole cell lysate was loaded as input.

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: HCT15

YTHDC2 Antibody Western Blot,Immunoprecipitation validation ( dilution) in HCT15 (Cat no:27779-1-AP)

FH

Robert (Verified Customer) (01-24-2019)

1. scrambled siRNA2. YTHDC2 siRNA12. YTHDC2 siRNA22. YTHDC2 siRNA3

• Applications: Western Blot,
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: ST cells (porcine)

YTHDC2 Antibody Western Blot, validation (1/500 dilution) in ST cells (porcine) (Cat no:27779-1-AP)