Tested Applications
| Positive WB detected in | BXPC-3 cells, HeLa cells, HepG2 cells, T47D cells, MCF-7 cells, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | rat testis tissue, human colon tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27779-1-AP targets YTHDC2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26690 Product name: Recombinant human YTHDC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1131-1430 aa of BC137285 Sequence: HSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSSADTEFSDECTTAERVLMKSPSPALHPPQKYKDRGILHPKRGTEDRSDQSSLKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPVRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSIVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPLVGEQLLQLWERLPLGEKNTTD Predict reactive species |
| Full Name | YTH domain containing 2 |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC137285 |
| Gene Symbol | YTHDC2 |
| Gene ID (NCBI) | 64848 |
| RRID | AB_2880970 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6S0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for YTHDC2 antibody 27779-1-AP | Download protocol |
| IF protocol for YTHDC2 antibody 27779-1-AP | Download protocol |
| IHC protocol for YTHDC2 antibody 27779-1-AP | Download protocol |
| IP protocol for YTHDC2 antibody 27779-1-AP | Download protocol |
| WB protocol for YTHDC2 antibody 27779-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The YTHDC2 Polyclonal Antibody (Cat# 27779-1-AP) has accumulated 54 citations published in journals including Nature Genetics, Nature Communications, Molecular Cell, Science Advances, Cell Reports, Cell Death & Disease, Advanced Science, Genes & Development, and FASEB Journal. Associated research fields encompass m6A RNA modification, oncology (pancreatic, colorectal, gastric, lung, breast, and liver cancers), neurology, immunology, metabolism, reproductive biology, cardiovascular disease, and toxicology. Cited applications span WB, IF, IHC, IP, CoIP, and RIP across human, mouse, rat, pig, and sheep.
| Species | Application | Title |
|---|---|---|
Nat Genet Super-enhancer RNA m6A promotes local chromatin accessibility and oncogene transcription in pancreatic ductal adenocarcinoma | ||
Cell Mol Immunol Inhibition of METTL3 in macrophages provides protection against intestinal inflammation | ||
Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis. | ||
Nat Commun USP5 stabilizes YTHDF1 to control cancer immune surveillance through mTORC1-mediated phosphorylation | ||
Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Genes Dis YTHDF1 shapes immune-mediated hepatitis via regulating inflammatory cell recruitment and response |
Reviews
What customers say
Customers report consistently strong performance across multiple applications. For Western blot, users note clean, strong signals at dilutions ranging from 1:500 to 1:4000, with minimal background. The antibody also performs well in immunohistochemistry and immunofluorescence (1:300) and in immunoprecipitation, where it successfully pulled down YTHDC2 from cell lysates. One user validated knockdown efficiency in porcine cells. Overall, reviewers rate the antibody highly, praising its specificity, low background, and reliability across WB, IHC, IF, and IP applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Liz (Verified Customer) (10-02-2025) | Very clean and strong signal
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Nikolett (Verified Customer) (07-23-2025) | I used this antibofy as 1:1000 for Western Blot, 1:300 for IHC and IF. Worked well without background, after overnight AB incubation in 3% BSA/PBS for WB. For IHC and IF I used NaCi pH6 for antigene retrieval and it worked nicely.
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Maialen (Verified Customer) (02-10-2022) | YTHDC2 was immunoprecipitated from 4 million HCT15 cells lysate using 1μg 27779-1-AP (YTHDC2 antibody). Same amount of normal rabbit IgG was used as negative control. For IP confirmation, western blot with 27779-1-AP (YTHDC2 antibody) was perfomed at 1:1000 dilution incubated overnight at 4ºC. 10% of the whole cell lysate was loaded as input.
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Robert (Verified Customer) (01-24-2019) | 1. scrambled siRNA2. YTHDC2 siRNA12. YTHDC2 siRNA22. YTHDC2 siRNA3
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