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YTHDF2 Antibody 20 Publications

Rabbit Polyclonal| Catalog number: 24744-1-AP

Featured Product KD/KO validated

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Con: 38 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

YTHDF2 Antibody WB Jurkat cells 24744-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

YTHDF2 Antibody WB Jurkat cells 24744-1-AP

WB result of YTHDF2 antibody (24744-1-AP, 1:4000) with si-control and si-YTHDF2 transfected Jurkat cells.

YTHDF2 Antibody IF mouse brain tissue 24744-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 24744-1-AP (YTHDF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

YTHDF2 Antibody IP Jurkat cells 24744-1-AP

IP Result of anti-YTHDF2 (IP:24744-1-AP, 4ug; Detection:24744-1-AP 1:600) with Jurkat cells lysate 2000ug.

YTHDF2 Antibody IHC human testis tissue 24744-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 24744-1-AP( YTHDF2 Antibody) at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, rat, swine
Positive WB detected in:
Jurkat cells, HeLa cells, L02 cells
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human testis tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue, HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
YTHDF2 fusion protein Ag5922
Full name:
YTH domain family, member 2
Calculated molecular weight:
62 kDa
Observed molecular weight:
62 kDa
GenBank accession number:
BC002559
Gene ID (NCBI):
51441
Gene symbol
YTHDF2
Synonyms
CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2

Background

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.


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