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- KD/KO Validated
YTHDF2 Polyclonal antibody
YTHDF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, RIP, IP, IHC, IF, CoIP, chIP,ELISA
Conjugate
Unconjugated
Cat no : 24744-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells, L02 cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | mouse testis tissue, human skeletal muscle tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse brain tissue, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 24744-1-AP is YTHDF2 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, swine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | YTHDF2 fusion protein Ag5922 |
Full Name | YTH domain family, member 2 |
Calculated molecular weight | 62 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC002559 |
Gene symbol | YTHDF2 |
Gene ID (NCBI) | 51441 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
IHC protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
IF protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
IP protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells. | ||
Oncogene Dynamic m6A mRNA methylation reveals the role of METTL3-m6A-CDCP1 signaling axis in chemical carcinogenesis.
| ||
Nucleic Acids Res Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation. | ||
Cell Stem Cell Targeting the RNA m6A Reader YTHDF2 Selectively Compromises Cancer Stem Cells in Acute Myeloid Leukemia.
| ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Robert (Verified Customer) (01-24-2019) | 1. scrambled siRNA 2. YTHDF2 siRNA
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