YTHDF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

YTHDF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, RIP, IP, IHC, IF, CoIP, chIP,ELISA

Conjugate

Unconjugated

Publications

62

Reviews (1)

Cat no : 24744-1-AP

Synonyms

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2

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Validation Data Gallery

• 

  WB analysis using 24744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24744-1-AP

  WB result of YTHDF2 antibody (24744-1-AP, 1:4000) with si-control and si-YTHDF2 transfected Jurkat cells. .

• 

  IF Staining of mouse brain using 24744-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 24744-1-AP (YTHDF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IHC staining of mouse testis using 24744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24744-1-AP (YTHDF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IP experiment of Jurkat using 24744-1-AP

  IP Result of anti-YTHDF2 (IP:24744-1-AP, 4ug; Detection:24744-1-AP 1:600) with Jurkat cells lysate 2000ug.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, L02 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse testis tissue, human skeletal muscle tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 20 publications below
• WB: See 51 publications below
• IHC: See 6 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 7 publications below

Product Information

The immunogen of 24744-1-AP is YTHDF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, swine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDF2 fusion protein Ag5922
• Full Name: YTH domain family, member 2
• Calculated molecular weight: 62 kDa
• Observed molecular weight: 62 kDa
• GenBank accession number: BC002559
• Gene symbol: YTHDF2
• Gene ID (NCBI): 51441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

Publications

Species	Application	Title
mouse	WB,IP	Nature; Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.; Authors - Dali Han
human	WB	Oncogene; Dynamic m6A mRNA methylation reveals the role of METTL3-m6A-CDCP1 signaling axis in chemical carcinogenesis.; Authors - Fan Yang ; KD Validated
mouse	WB	Int J Mol Sci; m6A Reader YTHDF2 Regulates LPS-Induced Inflammatory Response.; Authors - Ruiqing Yu ; KD Validated
human	WB	Nucleic Acids Res; Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation.; Authors - Tongxing Song
mouse	WB	Cell Stem Cell; Targeting the RNA m6A Reader YTHDF2 Selectively Compromises Cancer Stem Cells in Acute Myeloid Leukemia.; Authors - Jasmin Paris ; KD Validated
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Robert (Verified Customer) (01-24-2019)

1. scrambled siRNA 2. YTHDF2 siRNA

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

YTHDF2 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:24744-1-AP)

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