• Featured Product
  • KD/KO Validated

YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, RIP, IP, IHC, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 24744-1-AP

Synonyms

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, L02 cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse testis tissue, human skeletal muscle tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24744-1-AP is YTHDF2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF2 fusion protein Ag5922
Full Name YTH domain family, member 2
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC002559
Gene symbol YTHDF2
Gene ID (NCBI) 51441
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

Protocols

Product Specific Protocols
WB protocol for YTHDF2 antibody 24744-1-APDownload protocol
IHC protocol for YTHDF2 antibody 24744-1-APDownload protocol
IF protocol for YTHDF2 antibody 24744-1-APDownload protocol
IP protocol for YTHDF2 antibody 24744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Nature

Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.

Authors - Dali Han
humanWB

Oncogene

Dynamic m6A mRNA methylation reveals the role of METTL3-m6A-CDCP1 signaling axis in chemical carcinogenesis.

Authors - Fan Yang
  • KD Validated
mouseWB

Int J Mol Sci

m6A Reader YTHDF2 Regulates LPS-Induced Inflammatory Response.

Authors - Ruiqing Yu
  • KD Validated
humanWB

Nucleic Acids Res

Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation.

Authors - Tongxing Song
mouseWB

Cell Stem Cell

Targeting the RNA m6A Reader YTHDF2 Selectively Compromises Cancer Stem Cells in Acute Myeloid Leukemia.

Authors - Jasmin Paris
  • KD Validated
humanWB

Genes Dev

PMID: 30463905

Authors - Rosa M Rubio

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Robert (Verified Customer) (01-24-2019)

1. scrambled siRNA 2. YTHDF2 siRNA

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
YTHDF2 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:24744-1-AP)