YWHAH Recombinant antibody

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  WB analysis using 85987-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85987-1-RR (YWHAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

YWHAH Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85987-1-RR

Clone No.250473C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

Synonyms

14-3-3 eta, 14 3 3 protein eta, 14-3-3, 14-3-3 protein eta

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Tested Applications

• Positive WB detected in: HeLa cells, Raji cells, A431 cells, NIH/3T3 cells, fetal human brain tissue, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85987-1-RR targets YWHAH in applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YWHAH fusion protein Ag7435
• Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC003047
• Gene Symbol: YWHAH
• Gene ID (NCBI): 7533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q04917
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.