ZAK Recombinant antibody

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  WB analysis using 81161-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81161-2-RR (ZAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 81161-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81161-2-RR (ZAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 81161-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81161-2-RR against Human ZAK were performed. The affinity constant is 0.28 nM.

ZAK Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 81161-2-RR

Clone No.250559E6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AZK, EC:2.7.11.25, HCCS 4, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, Jurkat cells, MDA-MB-231 cells, HEK-293 cellls, U2OS cells, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81161-2-RR targets ZAK in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZAK fusion protein Ag30599
• Full Name: sterile alpha motif and leucine zipper containing kinase AZK
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 91kDa, 51 kDa
• GenBank Accession Number: BC001401
• Gene Symbol: ZAK
• Gene ID (NCBI): 51776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NYL2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38 gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa (ZAK alpha), 51 kDa (ZAK beta) and 35 kDa.