ZBTB4 Polyclonal antibody, PBS Only

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• ZBTB4 Polyclonal antibody, PBS Only (17611-1-PBS)

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  WB analysis using 17611-1-AP (same clone as 17611-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17611-1-AP (ZBTB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17611-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 17611-1-AP (same clone as 17611-1-PBS)

  WB result of ZBTB4 antibody (17611-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZBTB4 transfected PC-3 cells. This data was developed using the same antibody clone with 17611-1-PBS in a different storage buffer formulation.

ZBTB4 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 17611-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

KAISO L1, KAISO-L1, KAISO-like zinc finger protein 1, ZBTB 4, Zinc finger and BTB domain-containing protein 4

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Product Information

17611-1-PBS targets ZBTB4 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11774

  Product name: Recombinant human ZBTB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC043352

  Sequence: MPPPAEVTDPSHAPAVLRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLTSAPLPLPPATGGAAPNPATTTAASSSSSSSSSSSSSSSSASSSSSSSSSSPPPASPPASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPSTPAPMATSQPEEDSFGPGPRPAGEWEGDRAEAQAPDLQCSLPRRPLPCPQCGKSFIHPKRLQTHEAQCRRGASTRGSTGLGAGGAGPGGPAGVDASALPPPVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRT

• Full Name: zinc finger and BTB domain containing 4
• Calculated Molecular Weight: 1013 aa, 105 kDa
• Observed Molecular Weight: 105-140 kDa
• GenBank Accession Number: BC043352
• Gene Symbol: ZBTB4
• Gene ID (NCBI): 57659
• RRID: AB_3741925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P1Z0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZBTB4 (zinc finger and BTB domain containing 4), also known as KAISO-L1 (KAISO-like zinc finger protein 1), is a 1,013 amino acid nuclear protein that is involved in transcriptional regulation. ZBTB4 contains one BTB (POZ) domain, six C2H2-type zinc fingers and is phosphorylated and downregulated by HIPK2. The gene encoding ZBTB4 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.