ZC3H12A Polyclonal antibody

• Featured Product
• KD/KO Validated

ZC3H12A Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 25009-1-AP

Synonyms

MCPIP1, MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A

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Validation Data Gallery

• 

  WB analysis of K-562 using 25009-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 25009-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25009-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 25009-1-AP

  IP result of anti-ZC3H12A (IP:25009-1-AP, 4ug; Detection:25009-1-AP 1:300) with Raji cells lysate 2000ug.

• 

  IHC staining of human urothelial carcinoma using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse spleen tissue, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human urothelial carcinoma tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

25009-1-AP targets ZC3H12A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3H12A fusion protein Ag13877
• Full Name: zinc finger CCCH-type containing 12A
• Calculated Molecular Weight: 599 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC005001
• Gene Symbol: ZC3H12A
• Gene ID (NCBI): 80149
• RRID: AB_2879844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155.; Authors - Zhenzhen Li ; KD Validated
mouse	WB	Cell Physiol Biochem; Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.; Authors - Zhenzhen Li
mouse	IHC	J Immunol; MCPIP1/Regnase-1 Restricts IL-17A- and IL-17C-Dependent Skin Inflammation.; Authors - Leticia Monin
	WB	Retrovirology; Increased expression of CDKN1A/p21 in HIV-1 controllers is correlated with upregulation of ZC3H12A/MCPIP1.; Authors - Suwellen S D de Azevedo
mouse	IF	Neurosci Lett; TLR4 signaling pathway mediates the LPS/ischemia-induced expression of monocytechemotactic protein-induced protein 1 in microglia.; Authors - Shumin Chen
mouse	IF	Med Microbiol Immunol; MCPIP1 inhibits coxsackievirus B3 replication by targeting viral RNA and negatively regulates virus-induced inflammation.; Authors - Min Li