ZC3H12A Polyclonal antibody

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• ZC3H12A Polyclonal antibody (25009-1-AP)

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• KD/KO Validated

ZC3H12A Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 25009-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

MCPIP1, MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A

• 

  WB analysis of K-562 using 25009-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 25009-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 25009-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Raji using 25009-1-AP

  IP result of anti-ZC3H12A (IP:25009-1-AP, 4ug; Detection:25009-1-AP 1:300) with Raji cells lysate 2000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human urothelial carcinoma using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human urothelial carcinoma using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25009-1-AP (ZC3H12A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, mouse spleen tissue, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human urothelial carcinoma tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25009-1-AP targets ZC3H12A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13877

  Product name: Recombinant human ZC3H12A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 339-599 aa of BC005001

  Sequence: ANALLSPPRAPSKDKNGRRPSPSSQSSSLLTESEQCSLDGKKLGAQASPGSRQEGLTQTYAPSGRSLAPSGGSGSSFGPTDWLPQTLDSLPYVSQDCLDSGIGSLESQMSELWGVRGGGPGEPGPPRAPYTGYSPYGSELPATAAFSAFGRAMGAGHFSVPADYPPAPPAFPPREYWSEPYPLPPPTSVLQEPPVQSPGAGRSPWGRADSLAKEQASVYTKLCGVFPPHLVEAVMGRFPQLLDPQQLAAEILSYKSQHPSE

• Full Name: zinc finger CCCH-type containing 12A
• Calculated Molecular Weight: 599 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC005001
• Gene Symbol: ZC3H12A
• Gene ID (NCBI): 80149
• RRID: AB_2879844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5D1E8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.

Publications

What published studies show

The MCPIP1 (ZC3H12A/Regnase-1) affinity purification antibody (Cat# 25009-1-AP) has 14 total citations. Citations appear in journals including Cancer Discovery, International Immunopharmacology, Journal of Cellular Physiology, Journal of Immunology, Molecular Medicine Reports, Retrovirology, Chinese Medicine, Immunology Research, Cell Physiology and Biochemistry, Neuroscience Letters, Medical Microbiology and Immunology, and Biochemical Genetics. Research fields span immunology, inflammation, liver injury, neuroinflammation, oncology, infectious disease, and renal protection.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Discov; CD70-Targeted Allogeneic CAR T-Cell Therapy for Advanced Clear Cell Renal Cell Carcinoma; Authors - Sumanta K Pal
hamster	WB,IHC,IF	Chin Med; Based on the gut-heart axis: Polygonum capitatum improves atherosclerosis by modulating gut microbiota and TMAO, supporting MCPIP1/p53-associated endothelial protection.; Authors - Yunpei Wang ; KD Validated
human,mouse	WB	Int Immunopharmacol; MCPIP1 alleviates inflammatory response through inducing autophagy in Aspergillus fumigatus keratitis; Authors - Fang Han ; KD Validated
mouse	WB	Int Immunopharmacol; MCPIP1 modulates host antifungal immunity and exacerbates lethal Candida albicans infection.; Authors - Xueping Gao
mouse	WB,IF	Mol Med Rep; MCPIP1 alleviates depressive‑like behaviors in mice by inhibiting the TLR4/TRAF6/NF‑κB pathway to suppress neuroinflammation; Authors - Qi An
	WB	Retrovirology; Increased expression of CDKN1A/p21 in HIV-1 controllers is correlated with upregulation of ZC3H12A/MCPIP1.; Authors - Suwellen S D de Azevedo