ZC3H12A Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84521-5-RR (same clone as 84521-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84521-5-RR (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 84521-5-RR (same clone as 84521-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZC3H12A antibody (84521-5-RR, Clone: 241876H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84521-5-RR (same clone as 84521-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12A Recombinant antibody (84521-5-RR, Clone:241876H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84521-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84521-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84521-5-RR against Human ZC3H12A were performed. The affinity constant is 6.27 nM.

ZC3H12A Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84521-5-PBS

Clone No.241876H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

241876H5, EC:3.1.-.-, Endoribonuclease ZC3H12A, MCP induced protein 1, MCP-induced protein 1

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Product Information

84521-5-PBS targets ZC3H12A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13877

  Product name: Recombinant human ZC3H12A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 339-599 aa of BC005001

  Sequence: ANALLSPPRAPSKDKNGRRPSPSSQSSSLLTESEQCSLDGKKLGAQASPGSRQEGLTQTYAPSGRSLAPSGGSGSSFGPTDWLPQTLDSLPYVSQDCLDSGIGSLESQMSELWGVRGGGPGEPGPPRAPYTGYSPYGSELPATAAFSAFGRAMGAGHFSVPADYPPAPPAFPPREYWSEPYPLPPPTSVLQEPPVQSPGAGRSPWGRADSLAKEQASVYTKLCGVFPPHLVEAVMGRFPQLLDPQQLAAEILSYKSQHPSE

• Full Name: zinc finger CCCH-type containing 12A
• Calculated Molecular Weight: 599 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC005001
• Gene Symbol: ZC3H12A
• Gene ID (NCBI): 80149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5D1E8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.