ZC3H15 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZC3H15 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26241-1-AP

Synonyms

DFRP1, HT010, LEREPO4, MSTP012, PP730, ZC3H15

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Validation Data Gallery

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  WB analysis of K-562 using 26241-1-AP

  WB result of ZC3H15 antibody (26241-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H15 transfected K-562 cells.

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  WB analysis of HeLa using 26241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 26241-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26241-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 26241-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 26241-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 26241-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ZC3H15 antibody (26241-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 26241-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26241-1-AP (ZC3H15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, HL-60 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26241-1-AP targets ZC3H15 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3H15 fusion protein Ag24091
• Full Name: zinc finger CCCH-type containing 15
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC021102
• Gene Symbol: ZC3H15
• Gene ID (NCBI): 55854
• RRID: AB_2880441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.