ZC3H4 Polyclonal antibody

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  WB analysis of mouse brain using 20041-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20041-1-AP (ZC3H4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20041-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20041-1-AP (ZC3H4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 20041-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20041-1-AP (ZC3H4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 20041-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20041-1-AP (ZC3H4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20041-1-AP

  Immunofluorescent analysis of Hela cells, using ZC3H4 antibody 20041-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ZC3H4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20041-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C19orf7, KIAA1064, ZC3H4

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IF: See 3 publications below

Product Information

20041-1-AP targets ZC3H4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: zinc finger CCCH-type containing 4
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_015168
• Gene Symbol: ZC3H4
• Gene ID (NCBI): 23211
• RRID: AB_10667456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UPT8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H4, also named as C19orf7 and KIAA1064, is a 140kd protein. The antibody can recognizes near the C-term of ZC3H4.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Int J Biol Macromol; CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression; Authors - Zhongqiu Zhou
mouse	WB,IF	FASEB J; Silica-induced initiation of circular ZC3H4 RNA/ZC3H4 pathway promotes the pulmonary macrophage activation.; Authors - Xiyue Yang
human	WB	Cell Biosci; A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.; Authors - Juan Yin
mouse	WB	Lab Invest; Elk-1 transcriptionally regulates ZC3H4 expression to promote silica-induced epithelial-mesenchymal transition.; Authors - Rong Jiang
mouse	WB,IF	Toxicol Appl Pharmacol; ZC3H4 promotes pulmonary fibrosis via an ER stress-related positive feedback loop.; Authors - Hammad Ghafoor
human	WB,IF	Environ Toxicol Pharmacol; ZC3H4 mediates silica-induced EndoMT via ER stress and autophagy.; Authors - Rong Jiang