Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20041-1-AP targets ZC3H4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | zinc finger CCCH-type containing 4 |
| Calculated Molecular Weight | 140 kDa |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | NM_015168 |
| Gene Symbol | ZC3H4 |
| Gene ID (NCBI) | 23211 |
| RRID | AB_10667456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZC3H4, also named as C19orf7 and KIAA1064, is a 140kd protein. The antibody can recognizes near the C-term of ZC3H4.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| IHC protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| WB protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZC3H4 antibody (Cat# 20041-1-AP) has 11 citations published in journals including Cell, Molecular Cell, Cell Death & Disease, FASEB Journal, Laboratory Investigation, International Journal of Biological Macromolecules, Cell Biosciences, Environmental Toxicology and Pharmacology, Toxicology and Applied Pharmacology, and Toxicology Letters. Research fields span pulmonary fibrosis, epithelial-mesenchymal transition, innate immune signaling, mitochondrial complex I regulation, microglial apoptosis in depression, prostate stromal cell senescence, and anoikis resistance.
| Species | Application | Title |
|---|---|---|
Cell MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids | ||
Mol Cell ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts | ||
Cell Death Dis ZC3H4, a novel regulator of mitochondrial complex I, impacts prostate stromal cell senescence, attachment, adhesion and anoikis resistance. | ||
Int J Biol Macromol CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression | ||
Cell Biosci A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation. | ||
Environ Toxicol Pharmacol ZC3H4 governs epithelial cell migration through ROCK/p-PYK2/p-MLC2 pathway in silica-induced pulmonary fibrosis |
