ZCCHC8 Recombinant monoclonal antibody

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  WB analysis using 87017-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87017-1-RR (ZCCHC8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 87017-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZCCHC8 antibody (87017-1-RR, Clone: 252120B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity of 87017-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87017-1-RR against Human ZCCHC8 were performed. The affinity constant is 1.68 nM.

ZCCHC8 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87017-1-RR

Clone No.252120B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

TRAMP-like complex RNA-binding factor ZCCHC8, Zinc finger CCHC domain-containing protein 8

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87017-1-RR targets ZCCHC8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19985

  Product name: Recombinant human ZCCHC8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 355-707 aa of BC065918

  Sequence: KSVTYDLSKLVNYPGFNISTPRGIPDEWRIFGSIPMQACQQKDVFANYLTSNFQAPGVKSGNKRSSSHSSPGSPKKQKNESNSAGSPADMELDSDMEVPHGSQSSESFQFQPPLPPDTPPLPRGTPPPVFTPPLPKGTPPLTPSDSPQTRTASGAVDEDALTLEELEEQQRRIWAALEQAESVNSDSDVPVDTPLTGNSVASSPCPNELDLPVPEGKTSEKQTLDEPEVPEIFTKKSEAGHASSPDSEVTSLCQKEKAELAPVNTEGALLDNGSVVPNCDISNGGSQKLFPADTSPSTATKIHSPIPDMSKFATGITPFEFENMAESTGMYLRIRSLLKNSPRNQQKNKKASE

• Full Name: zinc finger, CCHC domain containing 8
• Calculated Molecular Weight: 707 aa, 79 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC065918
• Gene Symbol: ZCCHC8
• Gene ID (NCBI): 55596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6NZY4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. ZCCHC8 acts as a scaffold, mediating the interaction between RBM7 and MTR4. NEXT is involved in exosome-mediated surveillance and decay of noncoding RNAs, such as enhancer RNAs (eRNAs) and aberrant 3′-extended transcripts from small nuclear RNA (snRNA), telomerase RNA, and replication-dependent histone genes. NEXT is an important node in regulating nuclear exosome activities and takes part in several biological processes, including DNA damage response, stress response, and viral ribogenesis . ZCCHC8 also has a robust interaction with YTHDC1 (PMID: 28984244).