ZCRB1 Polyclonal antibody

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  WB analysis using 25629-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 25629-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25629-1-AP (ZCRB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZCRB1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25629-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Zinc finger CCHC-type and RNA-binding motif-containing protein 1, SNRNP31

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Tested Applications

• Positive WB detected in: ARPE-19 cells, Neuro-2a cells, Raji cells, SMMC-7721 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25629-1-AP targets ZCRB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22095

  Product name: Recombinant human ZCRB1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-217 aa of BC022543

  Sequence: MSGGLAPSKSTVYVSNLPFSLTNNDLYRIFSKYGKVVKVTIMKDKDTRKSKGVAFILFLDKDSAQNCTRAINNKQLFGRVIKASIAIDNGRAAEFIRRRNYFDKSKCYECGESGHLSYACPKNMLGEREPQKKKEKKKKKKAPEPEEEIEEVEESEDEGEDPALDSLSQAIAFQQAKIEEEQKKWKPSSGVPSTSDDSRRPRIKKSTYFSDEEELSD

• Full Name: zinc finger CCHC-type and RNA binding motif 1
• Calculated Molecular Weight: 217 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC022543
• Gene Symbol: ZCRB1
• Gene ID (NCBI): 85437
• RRID: AB_2880169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TBF4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Exp Clin Cancer Res; A novel protein encoded by ZCRB1-induced circHEATR5B suppresses aerobic glycolysis of GBM through phosphorylation of JMJD5.; Authors - Jian Song
human	WB	bioRxiv; Role of U11/U12 minor spliceosome gene ZCRB1 in Ciliogenesis and WNT Signaling; Authors - Geralle Powell-Rodgers