ZEB1 Recombinant antibody, PBS Only

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  WB analysis using 85729-1-RR (same clone as 85729-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85729-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 85729-1-RR (same clone as 85729-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85729-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 85729-1-RR (same clone as 85729-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85729-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85729-1-RR (same clone as 85729-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85729-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 85729-1-RR (same clone as 85729-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZEB1 antibody (85729-1-RR, Clone: 243139F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85729-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 85729-1-RR (same clone as 85729-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ZEB1 antibody (85729-1-RR, Clone: 243139F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85729-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85729-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85729-1-RR against Human ZEB1 were performed. The affinity constant is 0.693 nM.

ZEB1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85729-1-PBS

Clone No.243139F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8, TCF-8

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Product Information

85729-1-PBS targets ZEB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZEB1 fusion protein Ag16204
• Full Name: zinc finger E-box binding homeobox 1
• Calculated Molecular Weight: 1125 aa, 124 kDa
• Observed Molecular Weight: 190-210 kDa
• GenBank Accession Number: BC112392
• Gene Symbol: ZEB1
• Gene ID (NCBI): 6935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P37275
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa.