ZER1 Monoclonal antibody

ZER1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H9G7

Cat no : 68258-1-Ig

Synonyms

C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 68258-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68258-1-Ig (ZER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 68258-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68258-1-Ig (ZER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 68258-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZER1 antibody (68258-1-Ig, Clone: 1H9G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: LNCaP cells, Jurkat cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68258-1-Ig targets ZER1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZER1 fusion protein Ag10040
• Full Name: zer-1 homolog (C. elegans)
• Calculated Molecular Weight: 766 aa, 88 kDa
• Observed Molecular Weight: 80-88 kDa
• GenBank Accession Number: BC052563
• Gene Symbol: ZER1
• Gene ID (NCBI): 10444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.