ZFP36L2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 85154-4-RR (same clone as 85154-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36L2 antibody (85154-4-RR, Clone: 242776C5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85154-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85154-4-RR against Human ZFP36L2 were performed. The affinity constant is 2.38 nM.

ZFP36L2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85154-4-PBS

Clone No.242776C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2

---

Product Information

85154-4-PBS targets ZFP36L2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13515

  Product name: Recombinant human ZFP36L2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 155-497 aa of BC005010

  Sequence: KTELCRPFEESGTCKYGEKCQFAHGFHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNADERRPAPSGGASGDLRAFGTRDALHLGFPREPRPKLHHSLSFSGFPSGHHQPPGGLESPLLLDSPTSRTPPPPSCSSASSCSSSASSCSSASAASTPSGAPTCCASAAAAAAAALLYGTGGAEDLLAPGAPCAACSSASCANNAFAFGPELSSLITPLAIQTHNFAAVAAAAYYRSQQQQQQQQQQGLAPPAQPPAPPSATLPAGAAAPPSPPFSFQLPRRLSDSPVFDAPPSPPDSLSDRDSYLSGSLSSGSLSGSESPSLDPGRRLPIFSRLSISDD

• Full Name: zinc finger protein 36, C3H type-like 2
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC005010
• Gene Symbol: ZFP36L2
• Gene ID (NCBI): 678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47974
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZFP36L2 (Zinc finger protein 36 like 2) is an RNA-binding protein belonging to the ZFP36 family, which regulates gene expression at the post-transcriptional level by binding and destabilizing certain AU-rich elements (ARE) located in the 3′ untranslated regions (3′UTRs) of mRNA (PMID: 29518627). Moreover, ZFP36L2 is a key protein that controls S-phase progression in the case of genome instability (PMID: 29449217).