ZFPM1 Polyclonal antibody

ZFPM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28012-1-AP

Synonyms

FOG, FOG 1, FOG1, Friend of GATA 1, Friend of GATA protein 1, ZFPM1, Zinc finger protein ZFPM1, ZNF408, ZNF89A

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Validation Data Gallery

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  WB analysis of mouse liver using 28012-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 28012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 28012-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28012-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28012-1-AP targets ZFPM1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZFPM1 fusion protein Ag27829
• Full Name: zinc finger protein, multitype 1
• Calculated Molecular Weight: 105 aa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: NM_153813
• Gene Symbol: ZFPM1
• Gene ID (NCBI): 161882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFPM1 (Zinc Finger Protein, FOG Family Member 1, previous known as FOG1) is a protein with nine zinc finger domains, the C2HC type zinc fingers on ZFPM1 protein are responsible for mediating the interaction with the N-finger of GATA factors (PMID:10329627). ZFPM1 also contains two domains that are known to recruit transcriptional regulatory complexes. The one located at the extreme N-terminus is essential to recruit the nucleosome remodeling and histone deacetylase (NuRD) complex, another one between zinc fingers 6 and 7 could bind to CtBP co-repressor (PMID:30611731).